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KMID : 0360220160570030499
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Journal of the Korean Ophthalmological Society
2016 Volume.57 No. 3 p.499 ~ p.506
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Expression of Nestin on Endothelial Cells and Pericytes During Retinal Vascular Development in Mouse
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김진수:Kim Jin-Soo
박성욱:Park Sung-Wook/황인영:Hwang In-Young/김용우:Kim Yong-Woo/김진형:Kim Jin-Hyoung/김정훈:Kim Jeong-Hun
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Abstract
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Purpose: Nestin, a marker of neural stem cells, is expressed in Muller cells during retinal development. However, the role of nestin in retinal vascular development is not well established. Thus, we investigated the expression of nestin in developmental mouse retina and identified which retinal cells are related to the expression of nestin during the retinal vascular development.
Methods: Eyes were enucleated from C57BL/6 mice on postnatal day (P) 4, P8, P12, P16 and P26. Immunofluorescence was used to evaluate nestin expression in relation to endothelial cells (isolectin B4), pericytes (neural/glial antigen 2) and astrocytes (glial fibrillary acidic protein).
Results: Nestin was strongly expressed from the ganglion cell layer to retinoblast layer at P4. At P8, P12 and P16, the expression of nestin was observed from the upper border of the ganglion cell layer, and vertically penetrating to outer nuclear layer. At P26, the expression of nestin was decreased and confined to the ganglion cell layer and inner nuclear layer. Interestingly, there was strong vascular shape expression of nestin at all stages. The superficial, deep and intermediate vascular plexus was completely merged with nestin expression at P4, P8, P12 and P16. In addition, the nestin expression merged with pericytes but not with astrocytes.
Conclusions: Nestin was expressed in endothelial cells and pericytes during retinal vascular development in the retina. These results suggest that nestin could play an important role in developmental angiogenesis via interplay with endothelial cells and pericytes.
J Korean Ophthalmol Soc 2016;57(3):499-506
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KEYWORD
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Endothelial cell, Mouse retina, Nestin, Pericyte, Retinal vascular development
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